Fluorescence measurements - TCSPC

The fluorescence timing measurement is applied is numerous scientific and industrial applications, such as the characterization of molecules (proteins, peptides, lipids ...), ophthalmology, nanotechnology (carbon nanotubes ...) or the imaging microscopes.

This fluorescence signal is very low light intensity and measured by a very sensitive optical detector, a photon counter. This detector associated with a time correlator can reconstruct the fluorescence signal. The technique of Time Correlated Single Photon Counting (TCSPC) is particularly well suited for fluorescence measurements. Indeed, by adding small sample responses obtained over several cycles of excitation / emission, we obtain the fluorescence signal.

The latest photon counter with temporal correlation "all-in-one", the SPD_AT from AUREA Technology is the instrument best suited to measure time measurements of fluorescence using the TCSPC technique.